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Daita D. Ciobanu

Columbia University, New York, USA

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Born: 1977

Interests: molecular biology, protein biochemistry

Structural mechanics of ATP-driven polypeptide transport by SecA;
Site Directed Mutagenesis of the SecA Translocation ATPase. Location of the Trp Involved in the Signal Sequence Binding to E. coli SecA by Site Directed Mutagenesis. Laboratory of Prof. John F. Hunt. Department of Biological Sciences. at Columbia University , New York, NY, USA.

Selected publications:
• John J. Fak, Anna Itkin, Daita D. Ciobanu, Edward C. Lin, Xiang-Jin Song, Yi& #8209; Te Chou, Lila M. Gierasch, and John F. Hunt. Nucleotide Exchange from the High-Affinity ATP-Binding Site in SecA is the Rate-Limiting Step in the ATPase Cycle of the Soluble Enzyme and Occurs through a Specialized Conformational State. Biochemistry, 43(23);, pp. 7307-7327, 2004.
• Jordi Benach, Yi-Te Chou, John J. Fak, Anna Itkin, Daita D. Nicolae, Paul C. Smith, Guenther Wittrock, Daniel L. Floyd, Cyrus M. Golsaz, Lila M. Gierasch, and John F. Hunt. Phospholipid-induced Monomerization and Signal-peptide-induced Oligomerization of SecA. Journal of Biological Chemistry, 278 (6), pp. 3628-3638, 2003.


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