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Daniela Bratosin

Universitatea de Vest " Vasile Goldis" din Arad, Facultatea de Stiinte ale Naturii, Inginerie si Informatica, Arad, Romania

Institution web page: http://www.uvvg.ro/
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Born: 1952

Interests: apoptosis, flow cytometry, ageing, RBCs, cellular senescence, blood storage, toxi-and ecotoxicology

Details:
BRATOSIN DANIELA was born in November 1st 1952, Darmanesti-Bacau, and she graduated University of Bucharest, Faculty of Biology in 1976. In 1977 she graduated the Master of Microbiology-Genetics. She is Doctor of Biological Sciences - Romanian Academy since1995 and Professor of Ecotoxicology and Animal biology at University V Goldis of Arad since 2006.
She was Laureate of the Academy of Sciences in Paris - Charles Dhéré Prize for Biochemistry in 2002 and Laureate of the Romanian Academy-Nicolae Simionescu Prize for Biology, 2004
She had different fellowships: 1) FEBS-Advanced Course on Glycoconjugates, Villeneuve d'Ascq, France, 1986 ; 2)Short FEBS-fellowship ,Université des Science et Technologie de Lille I Laboratoire de Chimie Biologique (UMR no.111 al CNRS) Biomembranes (Directeur:Prof. Jean Montreuil), (3 month) 1990 ; 3) European Community Fellowship, PECO Go East- Go West Programe, Université des Science et Technologie de LilleI,Laboratoire de Chimie Biologique (UMR no.111 al CNRS) (Directeur: Prof. André Verbert), France 1993-1994 (10 month) ; 4)Post - doctoral MESR fellowship of French Ministry of University and Research (PECO-CEI Programe), Université des Science et Technologie de Lille I, Laboratoire de Chimie Biologique (UMR no.111 al CNRS) 1995-1996 (12 month) ; 5) 22 Research stages (3 month) financed by PECO-CEI Programe,Centre Regional de Transfusion Sanguine (CRTS) de Lille and Laboratoires Pharmaceutiques MacoPharma,France, 1996-2003.
Her main fields of research are : 1)Cellular and molecular mechanisms of cellular aging and apoptosis, particularly red cell senescence and erythrophagocytosis; 2)Cellular therapy: Improvement of storage conditions of blood in Blood Banks and of bone marrow and peripheral blood hematopoietic stem cell transplantation; 3)Stem Cell Research and Regenerative Medicine; 4) Development of biomarkers and cellular biosensors for molecular toxicology and ecotoxicology (nanotoxi and nanoecotoxicology); 5)Application for biomonitoring; 5) Development of Flow Cytometry methodologies for research and biotechnology fields; 6) Experimental models for biocompatibility analysis and flow cytometric assessment. Her scientific activity consist in : 1)one project financed by POS CCE Operation 2.1.2: " Complex research projects fostering the participation of high-level international experts"- structural founds 2) one international project of reserch (responsible) ; 2)15 projects of research (theme responsible for 5 of them) ; 3)28 scientific articles published in international journals (Cell Death and Differentiation, Cytometry, Journal of Immunological Methods, FEBS Letters, Glycoconjugate Journal, Biochimie, C. R. Acad. Sci., Paris, Vox Sanguinis, etc ) ; 4) 28 abstracts prezented at international scientific meetings and 10 abstracts at national meetings ; 5) 4 international patents ; 6) 1monograph in Romanian " Flow Cytometry for research, clinical and biotechnological aplications" (Explorarea structurii si functiilor celulare prin citometrie in flux), Vasile Goldis University Press, Arad, 2007 ; 7) teacher and invited speaker (plenary lectures) at over 10 international scientific meetings in French, Spain, Canada, Romania
She have agreements for international collaboration signed with: 1) Université des Science et Technologie de Lille , - Laboratoire de Glycobiologie Structurale et Fonctionnelle Unité mixte de Rechérche CNRS/USTL No 8576 Franta, Director:Prof. Jean Claude Michalski, Franta; 2)Universitaté Paris 7, Laboratoire EMI - U 9922 INSERM - Centre Hospitalier Bichat, Franta, Profesor Jean Claude Ameisen; 3) Les Laboratoires Pharmaceutiques MacoPharma, Tourcoing, Franta, Directeur F.Goudaliez

Selected publications:
• Bratosin D., Tissier J-P, Doummar D., Billette de Villemeur T., Cotoraci C., Hermine O., Montreuil J., Mignot C., . A cytometric study of the red blood cells in Gaucher disease reveals their abnormal shape that may be involved in increased erythrophagocytosis . Cytometry Part B (Clinical Cytometry), 80B, 1, pp. 28-37, 2011.
• Bratosin D, Mignot C, . In Memoriam Professor Emeritus Jean Montreuil (1920-2010) A Lifetime Dedicated to the Progress of Science and Education. Cytometry Part B (Clinical Cytometry), 80B , 2 , pp. 69-70 , 2011.
• 1. Bratosin D, Fagadar-Cosma E., Gheorghe A-M, Rugina A., Ardelean A., Montreuil J, Marinescu Al. G. ., In vitro toxi- and eco-toxicological assessment of porphyrine nanomaterials by flow cytometry using nucleated erythrocytes. Carpathian Journal of Earth and Environmental Sciences, , Vol. 6, , 2, p. 225 –, 2011.
• Bratosin D., Tissier JP., Doummar D., de Villemeur TB., Cotoraci C., Hermine O., Montreuil J., Mignot C. A focus on the red blood cells of Gaucher disease reveals their unexpected shape in some patients . In Cytomrtry Part B-Clinical Cytometry , 76B(6), pp. 424-425 . 2009.
• Bratosin D., Tcacenco L., Sidoroff M., Cotoraci C., Slomianny C., Estaquier J. and Montreuil J. . Active caspases - 8 and -3 in circulating human erythrocytes purified on immobilized Annexin-V. A cytometric demonstration. Cytometry Part A, , 75A, pp. 236-244, 2009.
• 3. Takacs-Buia L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D. Pathogenesis of osteoarthritis: Chondrocyte replicative senescence or apoptosis?. Cytometry Part B (Clinical Cytometry) , 74B, pp. 356-362, 2008.
• Mitrofan-Oprea L., Palii C., Tissier J-P., Héron A., Verpoort T., Behague M. , Goudaliez F., Smagghe E., Schooneman, F. Huart J-J., Montreuil J., Bratosin D. Novel criteria for assessing red blood cell viability in blood banks. Transfus. Clin. Biol. , 14, pp. 393-401, 2007.
• Bratosin D., Palii C., Moicean A. D., Zanetta J-P, Montreuil J., . Reduced diversity of the human erythrocyte membrane sialic acids in polycythemia vera and absence of N-glycolylneuraminic acid. Biochimie, 88,, p. 11,, 2006.
• Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J. Abstract Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. Cytometry A. , 66(1), pp. 78-84, 2005.
• Craciunescu O., Enescu DM., Bratosin D., Zarnescu O., Moldovan L., . Characterization of a biosubstitute containing elastin peptides. In Matrix Biology, 23, p. 397. ELSEVIER , 2004.
• Daniela Bratosin, Jérôme Estaquier, Christian Slomianny, Jean-Pierre Tissier, Brigitte Quatannens, Tatiana Bulai, Laura Mitrofan, Alexandru Marinescu, Ioana Trandaburu, Jean-Claude Ameisen and Jean Montreuil. On the evolution of erythrocyte programmed cell death: apoptosis of Rana esculenta nucleated red blood cells involves cysteine proteinase activation and mitochondrion permeabilization. . Biochimie, 86(3), pp. 183-192., 2004.
• Bulai T, Bratosin D, Artenie V, Montreuil J. . Uptake of sialic acid by human erythrocyte. Characterization of a transport system. . Biochimie, Jan;85(1-2):., pp. 241-244, 2003.
• Bulai T, Bratosin D, Pons A, Montreuil J, Zanetta JP. . Diversity of the human erythrocyte membrane sialic acids in relation with blood groups. FEBS Lett, 534(1-3), pp. 185-189, 2003.
• Bratosin D, Estaquier J, Ameisen JC, Montreuil J. . Molecular and cellular mechanisms of erythrocyte programmed cell death: impact on blood transfusion. . Vox Sang. , 83 Suppl 1, pp. 307-310, 2002.
• Bulai T, Bratosin D, Artenie V, Montreuil J. . Characterization of a sialate pyruvate-lyase in the cytosol of human erythrocytes. Biochimie, ;84(7):, pp. 655-660., 2002.
• Bratosin D, Estaquier J, Ameisen JC, Aminoff D, Montreuil J. . Flow cytometric approach to the study of erythrophagocytosis: evidence for an alternative immunoglobulin-independent pathway in agammaglobulinemic mice. . J Immunol Methods. , 265(1-2):, pp. 133-43., 2002.
• Bratosin D, Leszczynski S, Sartiaux C, Fontaine O, Descamps J, Huart JJ, Poplineau J, Goudaliez F, Aminoff D, Montreuil J. . Improved storage of erythrocytes by prior leukodepletion: Flow cytometric evaluation of stored erythrocytes. . Cytometry. , 15;46(6):, pp. 351-356., 2001.
• Bratosin D, Estaquier J, Petit F, Arnoult D, Quatannens B, Tissier JP, Slomianny C, Sartiaux C, Alonso C, Huart JJ, Montreuil J, Ameisen JC. . Programmed cell death in mature erythrocytes: a model for investigating death effector pathways operating in the absence of mitochondria. . Cell Death Differ. , 8(12), pp. 1143-1156, 2001.
• Bratosin D, Mazurier J, Tissier JP, Estaquier J, Huart JJ, Ameisen JC, Aminoff D, Montreuil J. . Cellular and molecular mechanisms of senescent erythrocyte phagocytosis by macrophages. A review. . Biochimie. , 80(2), pp. 173-195., 1998.
• Bratosin D, Mazurier J, Slomianny C, Aminoff D, Montreuil J. . Molecular mechanisms of erythrophagocytosis: flow cytometric quantitation of in vitro erythrocyte phagocytosis by macrophages. Cytometry. 1997 Oct 15; 30(5):269-74. , Oct 15;30(5):, pp. 269-274., 1997.
• Bratosin D, Mazurier J, Tissier JP, Slomianny C, Estaquier J, Russo-Marie F, Huart JJ, Freyssinet JM, Aminoff D, Ameisen JC, Montreuil J. Molecular mechanisms of erythrophagocytosis. Characterization of the senescent erythrocytes that are phagocytized by macrophages. . C R Acad Sci III, 1997.
• Bratosin D, Mazurier J, Debray H, Lecocq M, Boilly B, Alonso C, Moisei M, Motas C, Montreuil J. Flow cytofluorimetric analysis of young and senescent human erythrocytes probed with lectins. Evidence that sialic acids control their life span. . Glycoconj J. , Jun;12(3), pp. 258-267, 1995.
• Bratosin D., Motas C., . Effect of procaine and donor age on human erythrocyte-membrane - lectins binding study . Revue Roumaine de biochimie, 26(4), pp. 345-353, 1989.

Publications from the ISI database, indexed between 2002-2011, produced in Romania:
• Bulai, T; Bratosin, D; Artenie, V; Montreuil, J, Characterization of a sialate pyruvate-lyase in the cytosol of human erythrocytes. BIOCHIMIE, 84 (7), pp. 655-660, 2002.
• Bratosin, D; Estaquier, J; Ameisen, JC; Montreuil, J, Molecular and cellular mechanisms of erythrocyte programmed cell death: Impact on blood transfusion. VOX SANGUINIS, 83, pp. 307-310, 2002.
• Bratosin, D; Estaquier, J; Ameisen, JC; Aminoff, D; Montreuil, J, Flow cytometric approach to the study of erythrophagocytosis: evidence for an alternative immunoglobulin-independent pathway in agammaglobulinemic mice. JOURNAL OF IMMUNOLOGICAL METHODS, 265 (1-2), pp. 133-143, 2002.
• Bratosin, D; Leszczynski, S; Sartiaux, C; Fontaine, O; Descamps, J; Huart, JJ; Poplineau, J; Goudaliez, F; Aminoff, D; Montreuil, J, Improved storage of erythrocytes by prior leukodepletion: Flow cytometric evaluation of stored erythrocytes. CYTOMETRY, 46 (6), pp. 351-356, 2001.
• Bratosin, D; Estaquier, J; Petit, F; Arnoult, D; Quatannens, B; Tissier, JP; Slomianny, C; Sartiaux, C; Alonso, C; Huart, JJ; Montreuil, J; Ameisen, JC, Programmed cell death in mature erythrocytes: a model for investigating death effector pathways operating in the absence of mitochondria. CELL DEATH AND DIFFERENTIATION, 8 (12), pp. 1143-1156, 2001.
• Bulai, T; Bratosin, D; Artenie, V; Montreuil, J, Uptake of sialic acid by human erythrocyte. Characterization of a transport system. BIOCHIMIE, 85 (1-2), pp. 241-244, 2003.
• Bulai, T; Bratosin, D; Pons, A; Montreuil, J; Zanetta, JP, Diversity of the human erythrocyte membrane, sialic acids in relation with blood groups. FEBS LETTERS, 534 (1-3), pp. 185-189, 2003.
• Bratosin, D; Estaquier, J; Slomianny, C; Tissier, JP; Quatannens, B; Bulai, T; Mitrofan, L; Marinescu, A; Trandaburu, L; Ameisen, JC; Montreuil, J, On the evolution of erythrocyte programmed cell death: apoptosis of Rana esculenta nucleated red blood cells involves cysteine proteinase activation and mitochondrion permeabilization. BIOCHIMIE, 86 (3), pp. 183-192, 2004.
• Bratosin, D; Mitrofan, L; Palii, C; Estaquier, J; Montreuil, J, Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. CYTOMETRY PART A, 66A (1), pp. 78-84, 2005.
• Craciunescu, O; Enescu, DM; Bratosin, D; Zarnescu, O; Moldovan, L, Characterization of a biosubstitute containing elastin peptides. MATRIX BIOLOGY, 23 (6), p. 397, 2004.
• Bratosin, D; Palii, C; Moicean, AD; Zanetta, JP; Montreuil, J, Reduced diversity of the human erythrocyte membrane sialic acids in polycythemia vera. Absence of N-glycolylneuraminic acid and characterisation of N-acetylneuraminic acid 1,7 lactone. BIOCHIMIE, 89 (3), pp. 355-359, 2007.
• Mitrofan-Oprea, L; Palii, C; Tissier, JP; Heron, A; Verpoort, T; Behague, M; Smagghe, E; Schooneman, F; Huart, JJ; Goudaliez, F; Montreuil, J; Bratosin, D, Novel criteria for assessing red blood cell viability in blood banks. TRANSFUSION CLINIQUE ET BIOLOGIQUE, 14 (4), pp. 393-401, 2007.
• Takacs-Buia, L; Iordachel, C; Efimov, N; Caloianu, M; Montreuil, J; Bratosin, D, Pathogenesis of Osteoarthritis: Chondrocyte Replicative Senescence or Apoptosis?. CYTOMETRY PART B-CLINICAL CYTOMETRY, 74B (6), pp. 356-362, 2008.
• Bratosin, D; Tcacenco, L; Sidoroff, M; Cotoraci, C; Slomianny, C; Estaquier, J; Montreuil, J, Active Caspases-8 and-3 an Circulating Human Erythrocytes Purified on Immobilized Annexin-V: A Cytometric Demonstration. CYTOMETRY PART A, 75A (3), pp. 236-244, 2009.
• Bratosin, D; Tissier, JP; Doummar, D; de Villemeur, TB; Cotoraci, C; Hermine, O; Montreuil, J; Mignot, C, A FOCUS ON THE RED BLOOD CELLS OF GAUCHER DISEASE REVEALS THEIR UNEXPECTED SHAPE IN SOME PATIENTS. CYTOMETRY PART B-CLINICAL CYTOMETRY, 76B (6), pp. 424-425, 2009.
• Bratosin, D; Mignot, C, In Memoriam Professor Emeritus Jean Montreuil (1920-2010) A Lifetime Dedicated to the Progress of Science and Education. CYTOMETRY PART B-CLINICAL CYTOMETRY, 80B (2), pp. 69-70, 2011.
• Bratosin, D; Tissier, JP; Lapillonne, H; Hermine, O; de Villemeur, TB; Cotoraci, C; Montreuil, J; Mignot, C, A Cytometric Study of the Red Blood Cells in Gaucher Disease Reveals their Abnormal Shape that May Be Involved in Increased Erythrophagocytosis. CYTOMETRY PART B-CLINICAL CYTOMETRY, 80B (1), pp. 28-37, 2011.
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