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Apetri AC, Surewicz WK. . Kinetic intermediate in the folding of human prion protein. Journal of Biological Chemistry, 277(47), pp. 44589-92, 2002.

Abstract: Transmissible spongiform encephalopathies are associated with the conversion of cellular prion protein, PrP(C), into a misfolded oligomeric form, PrP(Sc). Here we have examined the kinetics of folding and unfolding reactions for the recombinant human prion protein C-terminal fragment 90-231 at pH 4.8 and 7.0. The stopped-flow data provide clear evidence for the population of an intermediate on the refolding pathway of the prion protein as indicated by a pronounced curvature in chevron plots and the presence of significant burst phase amplitude in the refolding kinetics. In addition to its role in the normal prion protein folding, this intermediate likely represents a crucial monomeric precursor of the pathogenic PrP(Sc) isoform.

Keywords: prion diseases, folding intermediate, ultra-fast kinetics, fluorescence spectroscopy


Posted by Adrian C Apetri


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