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Stef, Ducu-Sandu; Gergen, Iosif; Trasca, Teodor-Ioan; Rivis, Adrian; Stef, Lavinia; Cristina, Romeo Teodor; Druga, Marioara; Pet, Ioan. Assessing the influence of various factors on antioxidant activity of medicinal herbs. Romanian Biotechnological Letters, 22(4), pp. 12842-1284, 2017.

Abstract: The purposes of this study were: (1) to assess the antioxidant activity of fifteen medicinal plants using CUPRAC method, and (2) to highlight similarities or differences between results for the antioxidant activity estimated by FRAP and CUPRAC (cupric ion reducing antioxidant capacity) methods. The medicinal herbs have been purchased from the local market. The antioxidant activity of medicinal plants by CUPRAC method ranged from 34.20 mu mol of Trolox equivalents /g (Urtica dioica) and 512,48 mu mol of Trolox equivalents /g (Melissae Folium). The antioxidant capacity values after the CUPRAC method have been very high compared to those determined by FRAP method. The six herbs which recorded the highest antioxidant capacity by FRAP method are also found in the group of plants with very high antioxidant activity according with the CUPRAC method. In both cases the lowest antioxidant activity was assessed for Urtica dioica. Also, the last four positions were occupied by the same plants (Chelidonium majus, Capsella bursa pastoris, Valeriana officinalis and Urtica dioica) both for FRAP method and for the CUPRAC method. The hierarchies of medicinal plants, according to their antioxidant activities, were different for the two methods of measuring, respectively CUPRAC and FRAP.

Keywords: antioxidants; methods; diseases; FRAP; CUPRAC


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