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Romeo Teodor Cristina, Hanganu Flavia, Romeu Lazar, Adriana Constantin, Camelia Tulcan, Doru Morar, Ducu S. Stef, Hutu Ioan. . Prevalence of steroid hormone residues by GC-MS/MS screening in animal matrices in Romania. Romanian Biotechnological Letters , 22(1), pp. 12555-1256, 2017.

Abstract: The aim of this one year survey study was to assess, the prevalence of steroid hormones residues in the aim of National Residue Control Plan. GC-MS/MS in urine, serum, liver and muscle tissue in conformity with SOP ARO / 492-2006, respectively SOP ARO / 534-2008 was performed on 273 samples. Samples were purified using a one-step cleaning, detection being performed to a Varian 320-MS GC-MS/MS spectrophotometer (able to identify and confirm the matrix presence at 0.5 μ or 0.5 μg.L-1), the CCα values
for most compounds being between 0.1-0.3 μ or μg.L-1. The percentage of analyzed samples/species was: 40.29% poultry; 34.43%, cattle; 22.71% swine; 1.43% sheep and goats and respectively 1.09% equines. From total number of samples analyzed for detection of natural or synthetic steroid hormones/category, considered in this study, regardless of the matrix (urine, serum, and muscle tissue), 0.36% of samples were for medroxyprogesterone, 12.09% for estradiol, 31.5% for nortestosterone, respectively 56.05% for trenbolone. Samples analyzed / animal species were compliant for: medroxyprogesterone, estradiol, testosterone and trenbolone-cloud. In the case of estradiol, values identified were between 0.01-0.03 ppb for β fraction and of 0.01-0.06 ppb, for α fraction, but below detection limit of the method used.

Keywords: steroid hormones, residues, GC-MS/MS screening, animal matrices


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