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Aurelian Udristioiu. Diagnostic characterisation of rare diseases H2020-SC1-2016-2017 Project participant ; 2016-04-13; 2016-02-26 . 2016.

Abstract: Dr. Aurelian Udristioiu (AUDRISTIOIU2), looking for partner for my project of Doctoral These, entitled: Correlation between the cellular energy expressed in ATP and levels of mutant p53 protein in Chronic Lymphocytic Leukemia (2016-02-12) i on FP7or H2020, CORDIS. I wish to continue and develop fundamental research on the Onco-Hematology, "The correlation between the cellular bioenergetic expressed in ATP and concentatie levels of mutant p53 protein in CLL. In this regard we have prepared a summary on this issue that may put him at your disposal for verification and other data (CV, list of papers published in academic journals International and list of books written addressing issues hematologic and metabolic medicine Laboratory. High concentrations of anaerobic ATP implicated in aborted apoptosis from CLL ¹Udristioiu Aurelian, ²Cristina Florescu, ³Manuela Anda Radu-Popescu, 4 Manole Cojocaru ¹Emergency County Hospital TARGU-JIU,.Progresului Street No.18,Gorj, Postal Code:Zip 210218,Romania, ²Central Laboraotry Synevo, Clinical Trial, District 1, Bucharest, Romania ³UMF Carol Davila, Medicine Faculty, Department of Farmacy, Bucharest, Romania 4Titu Maiorescu University, Faculty of Medicine, Physiology Department, Bucharest, Romania ABSTRACT Apoptosis is an energy related process, in contrast with necrosis that occurs in the absence of adenosine-triphosphate (ATP). The main objective of the study was to assess intracellular ATP concentrations in B lymphocytes from patients with chronic lymphocytic leukemia (CLL), in comparison with ATP concentrations from B and T cells from patients with malignant diseases or non-malignant diseases, with allergic conditions. Material and method Using a Luminometer LKB analyzer, on principle of bioluminescence, we examined 75 patients (50 males and 25 females) with the following diagnoses: 25 patients (mean age 55 ± 0.5)) with allergic diseases (chronic allergic rhinitis, allergic dermatitis, chronic allergic asthma), 25 patients (mean age 58 ±1.8) with nonhematopoietc malignant diseases (lung cancer, bone metastasis) and 25 patients with confirmed diagnosis of CLL. Control group consisted of 120 healthy patients (mean ages 40.02 ±12.01). Results In the healthy male and female subjects, the mean concentration of ATP in 1 x 106 lymphocytes /ml of normal peripheral blood, T lymphocytes had was 1.39 µM ATP and in B lymphocytes the concentration was 0.35 µM ATP/ml [SD = 0.41, p= 0.030]. The mean concentration of ATP in 1 x 106 activated peripheral blood T lymphocytes from patients with allergic diseases was 3.12 µM ATP/ml and in B lymphocytes the mean concentration was 0.79 µM ATP/ml [SD= 0.56, p = 0.034]. From patients with malignant diseases, the concentration of ATP in 1 x 106 activated peripheral blood T lymphocytes/ml had a mean value of 3.06 µM ATP and the mean concentration of ATP in activated 1 x 106 peripheral blood B lymphocytes/ml was 0.17 µM ATP [SD= 0.45, p = 0.05]. The mean concentration of ATP in 1 x 106 activated peripheral blood malignant CLL B lymphocytes/ml was 4.33 µM ATP but was only 0.09 µM ATP [SD= 1.5, p< 0.05] in T lymphocytes from these patients. A strong correlation was observed between the concentration of ATP of T lymphocytes from patients with malignant diseases and ATP concentration of B lymphocytes from samples of patients with CLL (r= 0.99) and a good correlation was observed between T lymphocytes (Th) from allergic diseases and T lymphocytes (Ts) from malignant diseases (r = 0.94). Conclusion Blocked apoptosis from malignant diseases may be due to high ATP concentration originating from anaerobic metabolism. The difference of energy between anaerobic ATP in B lymphocytes from CLL and aerobic ATP in T lymphocytes from normal status in value of 2.68 µM ATP, as an energetic transfer between B and T cells, initiates carcinogenesis by suppression of anti oncogene proteins, especially p53 protein. Further studies are necessarY.

Keywords: Leukemia, anemia, cancer, carcinogenesis, protein p-53

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