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CRINA LAURA MOSNEANG, ADRIAN GROZEA, EUGENIA DUMITRESCU, FLORIN MUSELIN, ROMEO T. CRISTINA. A correlation between two different species of fish embryos used in a freshwater qualitative pollution test. Romanian Biotechnological Letters , 20, p. 2, 2015.

Abstract: Pollutants of freshwater sources, through their eco-risk potential can affect the human and animal health, numerous risk-assays being imagined and applied in the last decade. In this respect our aim was to make, an initial acute eco-risk study comparing the zebrafish embryos with an of interest autochthon species, namely pikeperch embryos, to see if this species is suitable and can be introduced as reliable testing tool in temperate region's water eco-pollution studies. The ISO 15088:2007 test method, adapted by our collective to pikeperch embryos stage development was used. Accordingly, 100 zebrafish embryos (Danio rerio) and 20 as control, in parallel with 100 pikeperch embryos (Sander lucioperca) and 20 control were kept in water samples provided from a swine farm surroundings, diluted in ten different concentrations: 1X, 2X, 3X, 4X, 6X, 8X, 12X, 16X, 24X, 32X. Any behaviour changes and mortality was recorded and statistically analysed according to Kolmogorov-Smirnov normality test, where P value was significant when P < 0.05. Though pikeperch embryo use for testing are not a pattern yet, as zebrafish embryos, results revealed that the indigene species embryos can be successfully used in studies applicable in ecotoxicology or other biomedical domains, with comparable results to the classical one.

Keywords: acute embryo-toxicity, pikeperch, zebrafish, risk-assay

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