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Augustin C. Mot, Cristina Bischin, Grigore Damian, Radu Silaghi-Dumitrescu. Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity. In Donald Armstrong (ed.), Methods in Molecular Biology, " Advanced Protocols in Oxidative Stress III" , Volume 1208, pp. 247-255. Spriger, 2015.

Abstract: Two methods for the measurement of antioxidant capacity are described: one based on a chronometric variation of a hemoglobin ascorbate peroxidase assay and the other based on electron paramagnetic resonance (EPR) spectra collected upon alkaline treatment of ethanolic samples. The involved chemical mechanisms are discussed, alongside the most important benefits and shortcomings; the assays offer new qualitative and quantitative information on samples of biological as well as synthetic origin.

Keywords: antioxidant activity assay, Hemoglobin ascorbate peroxidase assay, Electron Paramagnetic Resonance, Antioxidants, Natural extract, Polyphenolic compounds

Posted by Grigore DAMIAN


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