Ad Astra Awards
Ad Astra Journal
Science library
White book
University rankings
Who's who
Publications
Theses and dissertations
Ad Astra association
 
Press releases
News
Events
Funding opportunities
 
Login
Registration
 
>> Românã
 
   
 

Yan Li, Yuan Chen, Rong Xiang, Dragos Ciuparu, Lisa D. Pfefferle, Csaba Horvath, and James A. Wilkins. Incorporation of Single-Wall Carbon Nanotubes into an Organic Polymer Monolithic Stationary Phase for -HPLC and Capillary Electrochromatography. Analytical Chemistry, 77(5), pp. 1398-1406, 2005.

Abstract: Single-wall carbon nanotubes (SWNT) were incorporated into an organic polymer monolith containing vinylbenzyl chloride (VBC) and ethylene dimethacrylate (EDMA) to form a novel monolithic stationary phase for high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC). The retention behavior of neutral compounds on this poly(VBC-EDMA-SWNT) monolith was examined by separating a mixture of small organic molecules using micro-HPLC. The result indicated that incorporation of SWNT enhanced chromatographic retention of small neutral molecules in reversed-phase HPLC presumably because of their strongly hydrophobic characteristics. The stationary phase was formed inside a fused-silica capillary whose lumen was coated with covalently bound polyethyleneimine (PEI). The annular electroosmotic flow (EOF) generated by the PEI coating allowed peptide separation by CEC in the counterdirectional mode. Comparison of peptide separations on poly(VBC-EDMA-SWNT) and on poly(VBC-EDMA) with annular EOF generation revealed that the incorporation of SWNT into the monolithic stationary phase improved peak efficiency and influenced chromatographic retention. The structures of pretreated SWNT and poly(VBC-EDMA-SWNT) monolith were examined by high-resolution transmission electron microscopy, Raman spectroscopy, scanning electron microscopy, and multipoint BET nitrogen adsorption/desorption.

Keywords: PERFORMANCE LIQUID-CHROMATOGRAPHY, PEPTIDES, SEPARATION, PROTEINS, CO-MCM-41, COLUMNS, PURIFICATION

URL: http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/ac048299h

Posted by Dragos Ciuparu

Back

   
© Ad Astra 2001-2013