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Daniela Bratosin, Jérôme Estaquier, Christian Slomianny, Jean-Pierre Tissier, Brigitte Quatannens, Tatiana Bulai, Laura Mitrofan, Alexandru Marinescu, Ioana Trandaburu, Jean-Claude Ameisen and Jean Montreuil. On the evolution of erythrocyte programmed cell death: apoptosis of Rana esculenta nucleated red blood cells involves cysteine proteinase activation and mitochondrion permeabilization. . Biochimie, 86(3), pp. 183-192., 2004.

Abstract: Batracian Rana esculenta erythrocytes cell death induced by either calcium influx, or staurosporine, involves typical apoptotic phenotype. Our data reveal: (i) a drastic modification of the cell morphology with loss of the ellipsoidal form as assessed by phase contrast microscopy and scanning electron microscopy; (ii) an exposure of the phosphatidylserine residues in the outer leaflet of the cell membrane; (iii) a caspase-3-like activity; (iv) a mitochondrial membrane potential (DeltaPsim) loss; and (v) a chromatin condensation and fragmentation. Erythrocyte chromatin condensation and fragmentation are prevented by caspase and calpain peptide inhibitors. These inhibitors also prevent DeltaPsim loss supporting the idea that mitochondria is a central sensor for Rana erythrocytes cell death. Our observations highlight the conservation of the programmed cell death machinery in erythrocytes across kingdom.

Keywords: Apoptosis; Cell death; Red blood cells; Mitochondria; Calpain; Caspase; Amphibians

URL: /http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRJ-4C4X1N4-1&_coverDate=03%2F31%2F2004&_alid=258353639&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6236&_sort=d&view=c&_acct=C000036378&_version=1&_urlVersion=0&_userid=671124&md5=9e518a39d58cd7f7e006

Posted by Daniela Bratosin

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