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Ciolacu, Diana; Kovac, Janez; Kokol, Vanja, . The effect of the cellulose-binding domain from Clostridium cellulovorans on the supramolecular structure of cellulose fibers. CARBOHYDRATE RESEARCH, 345, pp. 621-630, 2010.

Abstract: The cellulose-binding domain (CBD) is the second important and the most wide-spread element of cellulase
structure involved in cellulose transformation with a great structural diversity and a range of
adsorption behavior toward different types of cellulosic materials. The effect of the CBD from Clostridium
cellulovorans on the supramolecular structure of three different sources of cellulose (cotton cellulose,
spruce dissolving pulp, and cellulose linters) was studied. Fourier-transform infrared spectroscopy (FTIR)
was used to record amides I and II absorption bands of cotton cellulose treated with CBD. Structural
changes as weakening and splitting of the hydrogen bonds within the cellulose chains after CBD adsorption
were observed. The decrease of relative crystallinity index of the treated celluloses was confirmed by
FTIR spectroscopy and X-ray diffraction (XRD). X-ray photoelectron spectroscopy (XPS) and scanning
electron microscopy (SEM) were used to confirm the binding of the CBD on the cellulose surface and
the changing of the cellulose morphology.

Keywords: Cellulose-binding domain, Cellulose supramolecular structure, FTIR, XRD, XPS, SEM

Posted by Diana Ciolacu


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