Ad Astra Awards
Ad Astra Journal
Science library
White book
University rankings
Who's who
Publications
Theses and dissertations
Ad Astra association
 
Press releases
News
Events
Funding opportunities
 
Login
Registration
 
>> Românã
 
   
 

Tata PR, Tata NR, Kühl M, Sirbu IO. . Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC. . Nucleic Acid Research, 2011.

Abstract: The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the transcription of the mouse EEmiRC locus, presumably through binding of transcription modulators like Oct3/4, Sox2 and CTCF. This IE also contains a CpG island showing a differential pattern of DNA and histone methylation marks during differentiation of ESCs, which places EEmiRC in a novel regulatory feedback loop with DNA methylases. Deletion of IE significantly reduced the transcription of the EEmiRC, further proving the importance of this region in regulating the expression of EEmiRC.

Keywords: MicroRNA, epigenetics, retinoic acid, pluripotency

URL: http://nar.oxfordjournals.org/content/early/2011/01/18/nar.gkq1344.long

Posted by Ioan Ovidiu Sirbu

Back

   
© Ad Astra 2001-2013